The CRISPR interference (CRISPRi) system has emerged as a robust tool for gene silencing, leveraging the precision of genome editing technologies to modulate gene expression without permanent genetic alterations. At the core of CRISPRi are custom sgRNA (single-guide RNA) vectors, which play a critical role in directing dead Cas9 (dCas9) to specific genomic loci to inhibit transcription...
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