Introduction to Single-Cell RNA Sequencing
RNA sequencing (RNA-seq) is a genomic technique for detecting and quantifying messenger RNA molecules in biological samples, which is useful for studying cellular responses. In recent years, RNA-seq has fueled a lot of medical discoveries and innovations. The approach is frequently applied to samples containing thousands to millions of cells for...
When a ribosome is affixed to the polypeptide (protein) it is making, the ribosome-nascent chain complex (RNC) is the compilation of molecules that make up the ribosome. One of several methods can be used to halt the synthesis of the nascent polypeptide. RNCs are made and purified in labs to study the dynamics, biochemistry, folding, and interactions that the ribosome and proteins undergoing...
What Is RNA Splicing?
RNA splicing is a post-transcriptional modification process that occurs in eukaryotic cells. When a gene is transcribed, the initial RNA product is called pre-mRNA, which contains both coding regions (exons) and non-coding regions (introns). RNA splicing removes the introns from the pre-mRNA molecule and joins together the exons to form a mature mRNA molecule that can be...
Introduction to CLIP Sequencing
RNA binding protein (RBP) is a key protein that regulates gene expression at the post-transcriptional level. RBP forms ribonucleoprotein complexes by binding to transcribed RNA. The complex can control RNA biogenesis, stability, cellular localization, and transport, as well as RNA molecules' fate and function. As a result, understanding post-transcriptional...
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